PHYTOCHEMICAL AND SOME PHARMACOLOGICAL STUDIES OF THE LEAF, STEM BARK AND ROOT BARK EXTRACTS OF STACHYTARPHETA ANGUSTIFOLIA (MILL) VAHL. (VERBENACEAE
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
ABSTRACT
The extracts of the leaf, stem bark and root bark of Stachytarpheta angustifolia (Mill) Vahl. (Verbenaceae), were subjected to preliminary phytochemical screening and the result revealed the presence of carbohydrates, steroids, cardiac glycosides, terpenoids, flavonoids, tannins and Saponins. Silica gel column chromatography of n- butanol soluble fractions of the leaf, stem bark and root bark extract followed by gel filtration using sephadex LH20 and preparative thin layer chromatography resulted in the isolation of two iridoid glycosides 1-[3,4-dihydroxy-5-(3-hydroxy-cycloprop-1-enyloxy)-6-hydroxymethyltetrahydro- pyran- 2-yloxy]- 7-methyl-1, 4a, 5, 6, 7, 7a hexahydrocyclopenta [c] pyran -4- carboxylic methyl ester and 4a, 7 – dihydroxy – 7 – methyl – 1 – (3, 4, 5 – trihydroxy – 6– hydro methyl – tetrahydropyran – 2 – yloxy) – 1 -, 4a, 5, 6, 7, 7a, hexahydro – cyclopentano [C] pyran – 4 – carboxylic acid methyl ester from the leaf and root bark respectively, while -β – (3‟, 4‟ – dihydroxy phenyl) – ethyl – O - - L – rhamnopyranosyl (1 3) – β – D – (4 – O - Caffeoyl) – glucopyranoside was isolated from the stem bark. The structures of these compounds were elucidated using chemical tests, spectroscopic techniques (FTIR, ID and 2D NMR) and by comparison with reference data. The ethanol leaf extract and its n-butanol and residual aqueous fractions exhibited concentration dependent contraction of the rabbit jejunum which was blocked by atropine, suggesting that the observed pharmacological effect were mediated through muscarinic receptors. In contrast, chloroform and Ethylacetate fraction of the ethanolic root bark extract exhibit concentration dependent relaxation of the rabbit jejunum. The ethanol leaf extract was screened for antimicrobial activity using disc diffusion and agar well dilution methods. The organisms tested were clinical isolates of S. aureus, S. pyogenes, E. coli and S. typhi. The ethanol extract inhibited the growth of all the microbes. The zone of inhibition ranging from 10-30mm). Conclusively, the use of S. angustifolia as a remedy for venerable infection by the folkloric healers has scientific basis .
Related Project Materials
ASSESSMENT OF WATER POVERTY IN MINNA, NIGERIA
ABSTRACT
Water is explicitly linked with economic progress and developmental trajectories of most countries and regions of the world. How...
BUDGETARY CONTROLS AND ITS LINK TO THE PERFORMANCE OF AN ORGANIZATION
ABSTRACT
Every organization doing exceedingly well on the face of the earth rely heavily on budgets, and budgetary control...
AN ASSESSMENT OF PHOTOGRAPHS AND CARTOONS AS COMMUNICATION INSTRUMENTS
ABSTRACT
Photographs in news stories can stimulate opinion change, either making a reader love the good advertised, or m...
ATTITUDE OF NURSES TOWARDS RELAPSE PREVENTION AMONG PSYCHIATRIC PATIENTS
ABSTRACT
The problem of every hospital when the targeted objective is no...
THE EFFECTS OF CONSUMERISM IN MARKETING OF FAKE & COUNTERFEIT DRUGS IN NIGERIA
Statement Of The Problem
In Nigeria, the pace at which business activities are increasing especially ma...
IMPLICATIONS OF LOCAL GOVERNMENT AUTONOMY ON RURAL DEVELOPMENT
Background of the study
Since time immemorial, local government autonomy has been a discussing issue among academi...
DESIGN AND IMPLEMENTATION OF ONLINE TUTORIAL ON FISH FARMING AND PRODUCTION
ABSTRACT
The tremendous impact of electronic technology (like computer and computer embedded devices, computer networkin...
A STATISTICAL ANALYSIS OF CAPITAL MARKET AND ECONOMIC GROWTH
ABSTRACT
This research work is aimed at establishing and testing for existing relationship between the...
A COMPARATIVE ANALYSIS OF RENTAL VARIATION IN RESIDENTIAL AND COMMERCIAL PROPERTIES IN NIGERIA
Abstract
This research has investigated in to a comparative analysis of rental variation in residential and commercial...
KNOWLEDGE AND ATTITUDE OF STUDENTS TOWARDS HIVAIDS
BACKGROUND TO THE STUDY
Sex is a basic physiological need of every human being, male and females are created with diffe...
